Journal: Frontiers in Veterinary Science

Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

doi: 10.3389/fvets.2024.1379146

Figure Lengend Snippet: Scatter plot of normalized miRNA expression in four canine lymphoma entities compared to PBMC as control group. The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (A) Group 3 = DLBCL vs. Control Group = PBMC; (B) Group 4 = PTCL (incl. Enteric TCL) vs. Control Group = PBMC; (C) Group 5 = TZL vs. Control Group = PBMC; (D) Group 6 = MZL vs. Control Group = PBMC.

Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

Techniques: Expressing, Control

Journal: Frontiers in Veterinary Science

Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

doi: 10.3389/fvets.2024.1379146

Figure Lengend Snippet: Fold regulation of significantly dysregulated miRNAs in four canine lymphoma entities by using PBMC as control group.

Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

Techniques: Control

Journal: Frontiers in Veterinary Science

Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

doi: 10.3389/fvets.2024.1379146

Figure Lengend Snippet: Normalized miRNA expression in two canine lymphoma entities. (A) Scatter plot of normalized miRNA expression comparing Group 3 (DLBCL) with Group 4 (PTCL, incl. Enteric TCL). The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (B) Volcano Plot illustrating significant miRNA expression changes when comparing Group 3 (DLBCL) with Group 4 (PTCL, incl. Enteric TCL). The center vertical line indicates unchanged miRNA expression, while the two outer vertical lines indicate the selected Fold regulation threshold. The horizontal line indicates the selected p -value threshold <0.05. MicroRNAs with data points in the far upper left and far upper right sections are down-regulated (green dots) or up-regulated (red dots) and meet the selected Fold regulation and p-value thresholds.

Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

Techniques: Expressing

Journal: Frontiers in Veterinary Science

Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

doi: 10.3389/fvets.2024.1379146

Figure Lengend Snippet: Scatter plot of normalized miRNA expression in four canine lymphoma entities compared to Lymph node (LN) as control group. The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (A) Group 3 = DLBCL vs. Control Group = LN; (B) Group 4 = PTCL (incl. Enteric TCL) vs. Control Group = LN; (C) Group 5 = TZL vs. Control Group = LN; (D) Group 6 = MZL vs. Control Group = LN.

Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

Techniques: Expressing, Control

Journal: Frontiers in Veterinary Science

Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

doi: 10.3389/fvets.2024.1379146

Figure Lengend Snippet: Fold regulation of significantly dysregulated miRNAs in four canine lymphoma entities by using Lymph node as control group.

Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

Techniques: Control

Journal: Frontiers in Veterinary Science

Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

doi: 10.3389/fvets.2024.1379146

Figure Lengend Snippet: DLBCL and PTCL (incl. Enteric TCL) miRNA fold regulations compared between the two control groups.

Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

Techniques: Control

Journal: Frontiers in Immunology

Article Title: Impact of the flame retardant 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) in THP-1 macrophage-like cell function via small extracellular vesicles

doi: 10.3389/fimmu.2022.1069207

Figure Lengend Snippet: Scatterplot of miRNA microarray analysis. CT data from samples (sEVs PBDE+LPS ) and control (sEVs DMSO+LPS ) were analyzed with the GeneGlobe miRCURY LNA miRNA PCR Data Analysis tool ( https://dataanalysis2.qiagen.com/miRCury ). MiRNAs were considered down-regulated if the fold change was <0.5 (green dot) and up-regulated if the fold change was >2 (red dots); in black are reported the unchanged miRNAs with respect to control (sEVs DMSO+LPS ). Normalization for the fold change calculation was performed using UniSp6 exogenous control and the geNorm (Predefined reference miRNA only) function.

Article Snippet: The CT data obtained from control and treated cells were analyzed using the Qiagen GeneGlobe miRCURY LNA miRNA PCR Data Analysis software tool, and the expression values were reported as fold change.

Techniques: Microarray, Control

Journal: Frontiers in Immunology

Article Title: Impact of the flame retardant 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) in THP-1 macrophage-like cell function via small extracellular vesicles

doi: 10.3389/fimmu.2022.1069207

Figure Lengend Snippet: Scatterplot of miRNA expression in M(0) THP-1 macrophage-like cells treated with sEVs PBDE+LPS . CT values obtained from three independent experiments (M(0) THP-1 macrophage-like treated with sEVs PBDE+LPS vs controls) were analyzed using the GeneGlobe miRCURY LNA miRNA PCR Data Analysis tool. Blue dots show down-regulated miRNAs (fold change <0.5), red is for up-regulated miRNA (fold change >2), and black indicates unchanged miRNAs with respect to control cells.

Article Snippet: The CT data obtained from control and treated cells were analyzed using the Qiagen GeneGlobe miRCURY LNA miRNA PCR Data Analysis software tool, and the expression values were reported as fold change.

Techniques: Expressing, Control